The author made calvarial defects of different sizes in rat to study the effect of defect size on healing process. Experimantal animals were sacrified at 3 days, 1 week, 2, 4, 6 weeks. The specimens were examed under light microscope with
Hematoxlin-Eosin and modified Gomori's trichrome stain, and under fluorescent microscope after glinding the specimens.
@ES The results were as follows.
@EN 1. 4mm-diameter calvarial defects healed about 70% after 6 weeks.
2. Lamellated bone trabecules were seen in 6mm-diameter calvarial defects after 4 weeks, and the small specules of bone and marrows were observed after 6 weeks.
3. 10mm-diameters calvarial defects healed about 50% and incomplete interconnection of the lamellated bone trabecules were observed after 6 weeks.
4. Under fluorescent microscope, about 4mm-long bone regeneration could be seen in most of the defects.
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